Suicide cancer gene therapy using pore-forming toxin, streptolysin O
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چکیده
منابع مشابه
Suicide cancer gene therapy using pore-forming toxin, streptolysin O.
We cloned the streptolysin O gene from the Streptococcus pyogenes genome and tested the possibility of using it as an anticancer reagent. Transient transfection of the streptolysin O gene efficiently killed 293T cells after 12 hours of transfection as determined by lactate dehydrogenase release and propidium iodide uptake. No caspase activity was observed and necrosis was prominent during strep...
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A major limitation of conventional chemotherapies used in cancer treatments today are low therapeutic indices and side effects that result from drug effects on normal tissues (off target). One of the most innovative approaches to developing antineoplastic agents with increased tumor selectivity is the use of suicide gene therapy. Suicide gene therapy involves delivering a gene product in proxim...
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ژورنال
عنوان ژورنال: Molecular Cancer Therapeutics
سال: 2006
ISSN: 1535-7163,1538-8514
DOI: 10.1158/1535-7163.mct-05-0515